A DNA analysis of the bacteria our group is attempting to use is important for several reasons: for comparing other iron reducers of the same genus; knowing what growing conditions are best, what products are likely to be produced; possibility of a new species; and (most importantly for me) to carry out further experiments on the plausibility of iron leaching.
Today was the third attempt at a PCR reaction and a third fail. All three trials were slightly different, in an attempt to adjust for the particular bacteria. Our next step will therefore be to isolate the DNA separately, then run a PCR. Hopefully that will assure DNA and we will be able to sequence after.
Well, I am off to try to call the vet before they close for the day: a friend's puppy tore her nail and she need to get it looked at. It's a very nasty tare, too. The tare does not go down (vertically) through the nail, but horizontally back into the nail. *sigh* Poor girl.
No comments:
Post a Comment