Tuesday, March 8, 2011

Third time's the charm? Not so much.

A DNA analysis of the bacteria our group is attempting to use is important for several reasons: for comparing other iron reducers of the same genus; knowing what growing conditions are best, what products are likely to be produced; possibility of a new species; and (most importantly for me) to carry out further experiments on the plausibility of iron leaching. 

Today was the third attempt at a PCR reaction and a third fail.  All three trials were slightly different, in an attempt to adjust for the particular bacteria.  Our next step will therefore be to isolate the DNA separately, then run a PCR.  Hopefully that will assure DNA and we will be able to sequence after.

Well,  I am off to try to call the vet before they close for the day: a friend's puppy tore her nail  and she need to get it looked at.  It's a very nasty tare, too.  The tare does not go down (vertically) through the nail, but horizontally back into the nail.  *sigh* Poor girl.

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