Thursday, March 17, 2011

DNA analysis attempt, again

First, no new developments seen in static flasks
Second, the bio grad student helping I'm working with for the PCR informed me that the reason we've been having so many problems is that the GoTaq was expired.  :-/  So, a PCR is being run right now, with new GoTaq.  Hopefully there are results this time around.  Since we have Gram-negative bacteria, they do not have a thick layer to get through, so it should work.  It'll be a great relief to see some bands this time in the gel.  The next step will be to sequence what we have, then compare that to a data base of known bacteria.  Here's hoping...

No comments:

Post a Comment